Fig. 7.

Relation of the MAPK pathway in accelerated HR cell death in NbPLC3s-silenced plants. Empty vector control and NbPLC3s-silenced plant leaves were infiltrated with Agrobacterium tumefaciens harboring 35S-MEK2^DD. (A) Cell death induction was determined by measuring the ion conductivity levels in control and NbPLC3s-silenced (PLC3s) plants. Values are means ±SD of four replicate experiments. (B) Total RNA was isolated from control and PLC3s plants at 0, 48, and 72 h after inoculation with A. tumefaciens harboring 35S-MEK2^DD. Expression values of Nbhin1 are shown as relative expression after normalization to internal standard genes (NbUbe35/NbNQO). Values represent means ± SD from triplicate experiments. Asterisks denote values significantly different from those of control plants (*; P<0.05, t-test). (C) MAPK activation in control and VIGS plants was analyzed by immunoblots with anti-P-p44/42 MAPK (T202/Y204) antibodies at 0, 24, and 48 h after inoculation. Protein loading was monitored by Ponceau S staining of the bands corresponding to ribulose-1,5-bisphosphate carboxylase large subunit.