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. 2023 May 16;74(15):4721–4735. doi: 10.1093/jxb/erad184

Fig. 9.

Fig. 9.

Role of the MAPK pathway in accelerated HR in NbPLC3s-silenced plants. Empty vector control, NbPLC3s-silenced (PLC3s), and NbPLC3s:NbMEK2 double-knockdown (PLC3/MEK2) plant leaves were infiltrated with R. solanacearum strain 8107. (A) Bacterial populations were determined in control, PLC3s, and PLC3/MEK2 plants by plating at 0, 18, and 24 h after inoculation. Values are means ±SD of four replicate experiments. Asterisks indicate significant differences among control, PLC3s, and PLC3/MEK2 plants (P<0.05, t-test). (B) Cell death induction was determined by measuring the ion conductivity levels in control, PLC3s, and PLC3s/MEK2 plants. Values are means ±SD of four replicate experiments. (D) Total RNA was isolated from control, PLC3s, and PLC3/MEK2 plants at 0, 15, and 18 h after inoculation with R. solanacearum 8107. Expression values of Nbhin1 are shown as relative expression after normalization to internal standard genes (NbUbe35/NbNQO). Values represent means ±SD from triplicate experiments. Asterisks denote values significantly different from those of control plants (*; P<0.05, t-test).