Figure 4.

IIV and LAIV elicit distinct BCR repertoires and transcriptional profiles

(A) Kinetics of HA⁺ B cells with a given transcriptional profile in tonsil organoids. Phenotypes were defined based on scRNA-seq and manual annotation of cluster identities. Activated, Act. 1, Act. 2, and GC-entry clusters. Memory, MemB, Act. MemB, and FCRL4 Pre-MemB clusters. GC, LZ and DZ-like clusters. PB, PB1 and PB2 clusters.

(B) BCR isotype usage in HA⁺ memory, GC, and PB B cells at different time points.

(C) BCR SHM frequencies of HA⁺ B cell subsets following organoid stimulation with IIV, LAIV, or H1N1. Unpaired Mann-Whitney U tests were used to compare groups; ∗p < 0.05 and ∗∗∗∗p < 0.0001.

(D) V gene usage of HA⁺ plasmablasts following stimulation with different influenza antigens on day 7.

(E) Shannon index as a metric of BCR diversity for HA+ plasmablasts. Boxplots show the median, with hinges indicating the first and third quartiles and whiskers indicating the highest and lowest value within 1.5 times the interquartile range of the hinges.

(F) Representative data (1 of 4 donors) of the number and size of HA⁺ BCR clonal families. Each wedge represents a clonal family. n = 4 donors (1 experiment) for all data. See also Table 1 and Figure S3.