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. 2023 Aug 8;56(8):1910–1926.e7. doi: 10.1016/j.immuni.2023.06.019

Figure 6.

Figure 6

Cellular and signaling mechanisms underlying differences in the quality and magnitude of the IIV vs. LAIV antibody response

(A–C) Type I interferon supplementation of immune organoids. Frequencies of HA+ B cells (A), HA+ plasmablasts (B), and influenza-specific antibodies (C) on day 7.

(D–F) Effects of naive or memory CD4 T cell depletion on organoid responses. Frequencies of total plasmablasts (D), HA+ B cells (E), and HA-specific Abs (F) on day 7.

(G–J) Effects of memory B cell depletion on organoid responses. Flow cytometry analysis of wild-type and memory B cell-depleted tonsil organoids on day 7 poststimulation (G). Antibody quantification (top) or fold-change (bottom) of specific antibodies on day 7 against hemagglutinin (H), neuraminidase (I), and nucleoprotein (J) in wild-type and memory B cell-depleted organoids. n = 6 donors combined from 2 experiments for all data shown. Boxplots show the median, with hinges indicating the first and third quartiles and whiskers indicating the highest and lowest value within 1.5 times the interquartile range of the hinges. ∗p < 0.05 and ∗∗p < 0.01 using a Kruskal-Wallis test followed by paired Mann-Whitney U tests to compare groups. p values shown are for comparisons against the unstimulated control unless otherwise indicated by lines. See also Tables 1 and S5.