TABLE 1.

Primers used for qRT PCR analysis of 10 target genes

Gene	Primer name	Sequence (5′–3′)	Length (bp)	Tm (°C)	Gene function	Reference
EF1	EF1_F2	AGTTCCAGGGGACAATGTTGG	21	59.8	Protein biosynthesis	33
	EF1_R2	TGGATTTCGCCAGGATGGTTC	21	59.8
CL112	aCL112_F2	GAAGCTACCAGATCCGTTTG G	21	59.8	Induction of strobilation	33
	aCL112_R2	TGCAAGCGCATCTGTTCACAG	21	59.8
CL355	CL355_for	TTCCGGAGAGCAGACCAATG	20	59.4	Induction of strobilation	42
	CL355_rev	CCAACGGCTGCATATACCATC	21	59.8
CL390	CL390_F2	AAGGTGCGACAATGAAGGTCC	21	59.8	Induction of strobilation	33
	CL390_R2	GTCTACAGGCTCAATGGTGTC	21	59.8
CL631	aCL631_F2	GCCTTGACGGTGAAAGATGAG	21	59.8	Induction of strobilation	33
	aCL631_R2	ACCTCGTCCTCATCCTTTTCG	21	59.8
DNMT	aDNMT_F2	CATGCACAGTTCATCGTCGAG	21	59.8	Role in methylation of DNA	33
	aDNMT_R2	CAATCGAACGCCACGTAATGC	21	59.8
RxR	aRXR_Q_F	AGATGTACAGTGCCACGTTGG	21	59.8	Induction of strobilation	33
	aRXR_Q_R	CAAGTGTTCGAGTGCTTGCAG	21	59.8
BMP	qBMP_F	GCAATGTAGCCCACTGCTTG	20	59.4	Secreted morphogen for cell differentiation and division	39
	qBMP_R	ATGCTTGGCCTTGGTCGAT	19	56.7
Wnt1	Wnt1_F	AGATTTCTGGCGCAAAAGGC	20	57.3	Morphogen for localization of anterior-posterior axis of strobilation segments	42
	Wnt1_R	TGTTTGCCTGCACCATTCAG	20	57.3
Ptc	Ptc_for	AGTGACAGCTCAACGAGTGG	20	59.4	Receptor in Shh signal pathway	42
	Ptc_rev	ACTCCGAGCAATGCTAACCA	20	57.3