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. 2023 Jul 20;11(4):e01667-23. doi: 10.1128/spectrum.01667-23

TABLE 1.

Glycan-binding profile of P. aeruginosa PAO1a

Glycan no. Glycan MFI for:
Array 1 Array 2 Array 3 Avg
1 β-Glc-Sp 156.625 376.875 413.625 315.708
2 β-Gal-Sp 271.125 313.75 347.75 310.875
3 α-Man-Sp 133.5 160.625 157.375 150.5
4 α-Fuc-Sp 180.375 327.125 307 271.5
5 α-Rha-Sp 163.625 135.375 115.375 138.125
6 β-GlcNAc-Sp 505.125 441.875 429 458.666
7 β-GalNAc-Sp 512.875 345.125 360.875 406.291
8 Tobramycin 485.25 449.75 432.375 455.791
9 Gal-β-1,3-GlcNAc-β-Sp 250 225.375 203.75 226.375
10 Gal-α-1,3-Gal-β-1,3-GlcNAc-β-Sp 187.125 375.625 337.5 300.083
11 Neu5Ac-α-2,3-Gal-β-1,3-GlcNAc-β-Sp 157.75 186 140.875 161.541
12 Neu5Ac-α-2,6-Gal-β-1,3-GlcNAc-β-Sp 10.625 57.375 55.75 41.25
13 Neu5Gc-α-2,3-Gal-β-1,3-GlcNAc-β-Sp 20 34 21.5 25.166
14 Neu5Gc-α-2,6-Gal-β-1,3-GlcNAc-β-Sp 19.375 63.25 72.5 51.708
15 Neu5Gc-α-2,6-Gal-β-1,3-GlcNAc-β-Sp 9 105.5 38 50.833
16 Gal-β-1,4-Glc-β-Sp 17 104.75 64 61.916
17 Gal-α-1,3-Gal-β-1,4-Glc-β-Sp 26.25 89.875 63.875 60
18 Gal-α-1,4-Gal-β-1,4-Glc-β-Sp 62.125 128 68.125 86.083
19 GlcNAc-β-1,3-Gal-β-1,4-Glc-β-Sp 5 76.625 62.375 48
20 GalNAc-β-1,3-Gal-β-1,4-Glc-β-Sp 148.375 168.375 161 159.25
21 Neu5Ac-α-2,3-Gal-β-1,4-Glc-β-Sp 75 79 61.875 71.958
22 Neu5Ac-α-2,6-Gal-β-1,4-Glc-β-Sp 71.625 46.75 59.375 59.25
23 Neu5Gc-α-2,3-Gal-β-1,4-Glc-β-Sp 6.5 80.125 116.125 67.583
24 Neu5Ac-α-2,6-Gal-β-1,4-Glc-β-Sp 59.75 84.25 110.5 84.833
25 Gal-β-1,4-(Fuc-α-1,3)-Glc-β-Sp 149.25 121.25 177.875 149.458
26 GalNAc-β-1,3-Gal-α-1,4-Gal-β-1,4-Glc-β-Sp 49 48.5 22.75 40.083
27 GlcNAc-β-1,6-GlcNAc-β-Sp 73.625 114.375 117.875 101.958
28 4-P-GlcNAc-β-1,4-Man-β-Sp 108 202.5 138.625 149.708
29 Glc-α-1,2-Gal-α-1,3-Glc-α-Sp 143.5 94.25 88.25 108.667
30 Gal-β-1,3-GalNAc-α-Sp 36.75 35.875 38 36.875
31 Gal-β-1,4-GlcNAc-β-Sp 6.125 40.875 20.75 22.583
32 Gal-β-1,4-(Fuc-α-1,3)-GlcNAc-β-[Lewis X]–Sp 30.375 65.5 35.625 43.833
33 Neu5Ac-α-2,3-Gal-β-1,4-(Fuc-α-1,3)-GlcNAc-β-[sialyl Lewis X]-Sp 20.875 38.625 26.25 28.583
34 Neu5Ac-α-2,3-Gal-β-1,3-(Fuc-α-1,4)-GlcNAc-β-[sialyl Lewis A]-Sp 15 16.875 12.875 14.916
35 Neu5Gc-α-2,3-Gal-β-1,3-(Fuc-α-1,4)-GlcNAc-β- [sialyl Lewis A]-Sp 13.125 11.75 2.25 9.0416
36 Gal-α-1,4-Gal-β-1,3-GlcNAc-β-Sp 12.625 46.375 38 32.333
37 Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-Glc-β-[LNnT]-Sp 193.75 183.625 133.375 170.25
38 GlcA-β-1,4-GlcNAc-α-1,4-GlcA-β-Sp 122.25 115.25 89.625 109.041
39 GlcNAc-β-1,6-(Gal-β-1,3)-GalNAc-α-OSer-Sp4 34.625 44 8.25 28.958
40 Neu5Ac-α-2,3Gal-β-1,4-(6S)GlcNAc-β-Sp 28.25 36.25 55 39.833
41 GalNAc-β-1,4-GlcNAc-β-Sp2 110.625 103.875 86 100.166
42 Neu5Ac-α-2,8-Neu5Ac-α-2,3-Gal-β-1,4-Glc-β-Sp 65.625 56.125 27.75 49.833
43 Neu5Gc-α-2,8-Neu5Ac-α-2,3-Gal-β-1,4-Glc-β-Sp 16.25 37.5 50.875 34.875
44 GalNAc-α-1,3-(Fuc-α-1,2)-Gal-β-1,4-Glc-β-[blood A antigen tetrose]-Sp1 73.75 76.75 72.875 74.458
45 GlcNAc-β-1,2-Man-α-Sp 26.25 30 39.375 31.875
46 Neu5Ac-α-2,3-Gal-β-Sp1 10.25 35.125 27 24.125
47 Gal-β-1,3-GalNAc-β-1,3-Gal-β-Sp1 17.25 39.75 46.875 34.625
48 Glc-α-1,2-Gal-α-Sp 160.875 89.875 90.375 113.708
49 Gal-β-1,4-(Fuc-α-1,3)-GlcNAc-β-1,3-Gal-β-Sp1 5.75 60.875 52.5 39.708
50 Neu5Ac-α-2,3-Gal-β-1,4-(Fuc-α-1,3)-Glc-β-[3-sialyl-3-fucosyllactose/F-SL]-Sp1 28.625 74.875 57.75 53.75
51 GlcNAc-β-1,4-GlcNAc-β-Sp1 57 68.125 58.125 61.083
52 β-d-GlcA-Sp 23 132.625 107.25 87.625
53 Gal-β-1,4-(6S)GlcNAc-β-Sp 101 156.875 103.125 120.333
54 GlcNAc-α-1,3-(Glc-α-1,2-Glc-α-1,2)-Gal-α-1,3-Glc-α-Sp 57.375 31 47.625 45.3333
55 Gal-β-1,3-GalNAc-β-1,4-(Neu5Gc-α-2,3)-Gal-β-1,4-Glc-β-Sp1 6.875 98.125 95.875 66.958
56 Sisomicin sulfate 17.375 54.625 16.625 29.541
57 GalNAc-α-1,3-(Fuc-α-1,2)-Gal-β-[blood A antigen trisaccharide]-Sp1 163.75 154.625 123.75 147.375
58 Fuc-α-1,2-Gal-β-1,4-GlcNAc-β-[blood H antigen trisaccharide]-Sp1 68.125 94.875 62.625 75.208
59 Gal-α-1,3-(Fuc-α-1,2)-Gal-β-[blood B antigen trisaccharide]-Sp1 124.875 139.5 119.125 127.833
60 Fuc-α-1,2-Gal-β-1,3-GlcNAc-β-1,3-Gal-β-1,4-Glc-β-[LNFP I]-Sp1 52.5 84.25 104.375 80.375
61 Fuc-α-1,2-Gal-β-1,4-Glc-β- [blood H antigen trisaccharide]-Sp1 48.75 33.25 36.25 39.416
62 Gal-α-1,3-(Fuc-α-1,2)-Gal-β-1,4-Glc-β-[blood B antigen tetrasaccharide]-Sp1 18.75 25.75 27.375 23.958
63 (Fuc-α-1,2)-Gal-β-1,4-(Fuc-α-1,3)-GlcNAc-β-[Lewis Y]-Sp1 1.25 23.625 8.875 11.25
64 (Fuc-α-1,2)-Gal-β-1,3-(Fuc-α-1,4)-GlcNAc-β-[Lewis B]-Sp1 12.5 29.75 6.125 16.125
65 Gal-β-1,3-(Fuc-α-1,4)-GlcNAc-β-1,3-Gal-β-1,4-(Fuc-α-1,4)-Glc-β-[Lewis A]-Sp1 19.75 42.625 9 23.791
66 Gal-β-1,3-GalNAc-β-Sp1 14.625 18 30.125 20.916
67 Gal-β-1,3-(Neu5Ac-α-2,6)-GalNAc-β-Sp 3.25 4.5 14.75 7.5
68 Neu5Ac-α-2,6-Gal-β-1,3-GalNAc-β-Sp 42.125 27.625 39.125 36.291
69 Neu5Ac-α-2,6-Gal-β-1,3-(Neu5Ac-α-2,6)-GalNAc-β-Sp 53.875 56.625 45.5 52
70 Neu5Ac-α-2,3-Gal-β-1,3-(Neu5Ac-α-2,6)-GalNAc-β-Sp 70.25 93.25 21 61.5
71 Neu5Ac-α-2,6-(Neu5Ac-α-2,3)-Gal-β-1,3-GalNAc-β-Sp 4.625 5 1.25 3.625
72 GalNAc-β-1,4-(Neu5Ac-α-2,3)-Gal-β-1,4-Glc-β-[GM2]-Sp 4.5 37.25 75.625 39.125
73 GalNAc-β-1,4-(Neu5Ac-α-2,8-Neu5Ac-α-2,3)-Gal-β-1,4-Glc-β-[GD2]-Sp 31 49.75 17.625 32.791
74 Gal-α-1,4-Gal-β-1,4-GlcNAc-β-Sp1 92 27.625 87 68.875
75 β-d-Rha-Sp 254.875 294.375 263 270.75
76 Glc-α-1,4-Glc-β-Sp1 120.5 274.25 264.125 219.625
77 Glc-α-1,6-Glc-α-1,4-Glc-β-Sp1 157.125 132.125 75.75 121.666
78 Maltotriose-β-Sp1 52.5 100.5 26.875 59.958
79 Glc-α-1,6-Glc-α-1,6-Glc-β-Sp1 11 90.875 60.375 54.083
80 Maltotetraose-β-Sp1 89 71.5 63.5 74.666
81 GlcNAc-α-1,4-GlcA-β-1,4-GlcNAc-α1,4-GlcA-β-Sp 36.5 62.375 61.25 53.375
82 Maltohexaose-β-Sp1 8.5 44.875 24 25.791
83 Maltoheptaose-β-Sp1 28.625 47.5 28.875 35
84 Acarbose-β-Sp1 39.5 75 60.25 58.25
85 d-Pentamannuronic acid-β-Sp1 55 248.625 179.625 161.083
86 l-Pentaguluronic acid-β-Sp1 50.25 105.75 103.625 86.541
87 d-Cellose-β-Sp1 5 79.25 1.75 28.666
88 Gal-α-1,3-Gal-β-Sp1 26 153.5 118.5 99.333
89 β-1,4-xylotetrose-Sp1 42 122.25 94.125 86.125
90 Chitin-trisaccharide-Sp1 31.875 21.75 24.125 25.916
91 KDN-α-2,8-Neu5Ac-α-2,3-Gal-β-1,4-Glc-β-Sp 153.375 132 41 108.791
92 Neu5Ac-α-2,8-Neu5Gc-α-2,3-Gal-β-1,4- Glc-β-Sp 21.25 40.25 23.75 28.416
93 Neu5Ac-α-2,8-Neu5Ac-α-2,8-Neu5Ac-α-2,3-Gal-β-1,4-Glc-β-Sp3 15.875 68.125 40.125 41.375
94 Neu5Ac-α-2,8-Neu5Ac-α-2,6-Gal-β-1,4-Glc-Sp5 12.75 43.5 4.25 20.166
95 Gal-β-1,3-GalNAc-β-1,4-(Neu5Ac-α-2,3)-Gal-β-1,4-Glc-β-Sp1 21.5 105.5 54.125 60.375
96 Gentamicin sulfate 16.625 64.25 91 57.291
97 Kanamycin sulfate 30.5 66.75 142.75 80
98 Geneticin disulfate salt (G418) 12.25 36.5 37.75 28.833
99 Neomycin trisulfate 25.375 111.625 34.875 57.291
100 SGP 74.5 60.375 101.25 78.708
a

Glycan identities and mean fluorescence intensities (MFIs) of GFP-expressing P. aeruginosa PAO1 cells binding to 100 immobilized glycans. MFIs for each array were calculated as the average of the four data points from each glycan, spotted in quadruplicate on each array. A composite average MFI from the data across the 3 arrays (n = 12 for each glycan moiety) is shown in the final column. Positive glycan partners for P. aeruginosa were identified by the MFI as being at least 100 RFUs over background and significantly greater than the negative control; 28 glycans met these criteria.