Circulating and tumor-infiltrating lymphocyte composition. (A) Frequency of CD19+ B cells and CD3+ T cells. (B) Frequency of CD4+ T cells, CD4+ naive T cells (CD45RA+ CCR7+), and effector memory (CD45RA+ CCR7−) subsets. (C) Frequency of CD8+ T cells, CD8+ naive T cells (CD45RA+ CCR7+), and effector memory (CD45RA+ CCR7−) subsets. (D) Frequency (%) of CD20+ B cells and CD3+ T cells in precancer and cancer groups performed through immunohistochemistry analysis. H/PB, healthy control, peripheral blood; PC/PB, precancer, peripheral blood; C/PB, cancer, peripheral blood; C/TT, cancer, tumor tissue. Statistical analysis was done by one way ANOVA followed by the Kruskal-Wallis test. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001.