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Expression of l,d-transpeptidase-mediated resistance in (p)ppGpp-producing E. colia
| Plasmid | Inhibition zone size (mm) around disks containing indicated β-lactam |
|
|---|---|---|
| Ceftriaxone (30 μg) | Ampicillin (10 μg) | |
| pHV6 (vector) | 37 | 23 |
| pHV6 (ycbB) | 12 | ≤6 |
| pHV6 (ynhG) | 38 | 21 |
| pHV6 (ldtPae1) | 39 | 20 |
The E. coli host harbored a chromosomal deletion of relA and plasmid pKT8 encoding RelA′. Data are median values of three biological repeats.
