TABLE 2.
Determination of the optimal coating amount of S268 protein and the optimal dilution of HEV-Nb67-HRP fusions using direct ELISA
| Amt of HEV-ORF2C protein (μg/mL) | Dilution of HEV-Nb67-HRP fusions in medium |
|||||||
|---|---|---|---|---|---|---|---|---|
| 1:24 | 1:25 | 1:26 | 1:27 | 1:28 | 1:29 | 1:210 | 1:211 | |
| 4 | 2.664 | 2.601 | 2.499 | 2.203 | 2.042 | 1.502 | 1.028 | 0.541 |
| 2 | 2.421 | 2.372 | 2.2 | 1.966 | 1.439 | 0.844 | 0.493 | 0.261 |
| 1 | 2.224 | 1.874 | 1.77 | 1.448 | 0.989 a | 0.601 | 0.3 | 0.179 |
| 0.5 | 1.958 | 1.446 | 1.119 | 0.855 | 0.484 | 0.227 | 0.14 | 0.084 |
a
The optimal amount of S268 protein and dilution of HEV-Nb67-HRP were selected when the OD450 value of the direct ELISA was approximately 1.0.