FIG 2.

The C terminus of 1835 has antibacterial activity. (A) Schematic diagram of the 1835 protein. The PAAR region of the peptide is shown in yellow, the middle fragment is shown in blue, and the C39 peptide region is shown in purple. (B) Growth of 10× serial dilutions of bacteria on Luria Bertani agar (LBA) plates and on LBA plates containing 50 μg/mL kanamycin and 0.5 mM isopropyl-β-d-thiogalactopyranoside (IPTG). The negative control is BL21(DE3) containing an empty PET28a plasmid. The positive control is Rhs-3CT in ExPEC PCN033.