FIG 7.

Tsap peptides exhibit preferential binding to S. aureus LTA. The ITC results of LPS binding to Tsap-1 (A), Tsap-2 (B) and Tsap-3 (C). ITC results for Tsap-1 (D), Tsap-2 (E), and Tsap-3 (F) and LTA are shown. The original titration data and the integrated heat measurements are shown in the left and right plots, respectively. Competitive bactericidal activity of Tsap-1 (G), Tsap-2 (H), and Tsap-3 (I) between LPS and LTA. S. aureus ATCC 25923 cells were grown to mid-log phase, washed three times with PBS and adjusted to an OD₆₀₀ of 1.0. LPS (1 mg/mL) and LTA (1 mg/mL) were added separately, and various concentrations of AMPs were added. After incubation at 37°C for 2 h, 10× serial dilutions were prepared, and CFU were counted.