FIG 3.

Abx intervention affected intestinal barrier function, while QYD treatment increased intestinal barrier protein expression and decreased intestinal permeability. (A) Representative H&E staining of intestine samples via microscope (100× and 200× magnification) and transmission electron microscopy (12,000× and ×20,000 magnification). L: lysosomal, M: mitochondria, Tj: tight junction. (B) The pathological score of the intestine (ileum). (C) Representative Western blotting images of ZO-1 and occludin. (D) Relative expression ratio of ZO-1/β-actin and occluding/β-actin. (E to G) Concentrations of d-LAC, LPS, and DAO in serum. (H) Immunofluorescence staining results for ZO-1 (red), occludin (green), and DAPI (blue), as well as merged images. Scale bar: 100 μm. (I) Heatmap of the correlations between intestinal bacteria (genus level) and pathological indices of SAP-ALI based on Spearman's correlation analysis. PS: pathological score. Data are representative images or shown as mean ± SEM for each group of mice (n = 7 per group) with at least three independent experiments and analyzed by unpaired Student's t test. *, P < 0.05; **, P < 0.01; ***, P < 0.001 in comparison with the CON group and ^#, P < 0.05; ^##, P < 0.01; ^###, P < 0.001 in comparison with the SAP group.