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. 2023 Jun 26;11(4):e00186-23. doi: 10.1128/spectrum.00186-23

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Copyright © 2023 Zhang et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 2 — Conserved domains and phylogenetic analysis of LaeA in different fungi. (A) Homologous sequences of LaeA in different fungi were retrieved from the NCBI database, and the conserved domains were analyzed using BioEdit software. The GenBank accession numbers of the homologous sequences of LaeA in nematode-trapping fungi A. oligospora, D. haptotyla, D. brochopaga, and D. cylindrospora are XP_011127052.1, EPS40087.1, KAF3913920.1, and KAF3939157.1, respectively. The GenBank accession numbers of these LaeA proteins are shown in the figure. Red triangles indicate a conserved SAM binding site, and the logo of the conserved domain was predicted using MEME online software. (B) Phylogenetic tree constructed by MEGA X software using the neighbor-joining method. Bootstrap values based on 1,000 replicates are shown in the phylogenetic tree, and AfLaeA (DFL_005107) is highlighted in red.