FIG 2.

Potentially relevant influence factors and pathway enrichment analyses for gastric metabolite alterations after H. pylori eradication. (A) Paired plots showed significant decreasing trends of DOB values (P < 0.001), pH values (P = 0.001), and metabolic indexes (P < 0.001) and significant increasing trends of microbial diversity indexes (P < 0.001 for both) after successful H. pylori eradication. However, in the failed treatment group, except that pH values were increased significantly (P = 0.020), the factors showed no significant alterations (P > 0.05 for all). a, metabolic index was calculated using the 81 differential metabolites after H. pylori eradication to represent the overall metabolic status in each gastric biopsy specimen by the logistic regression equation in Materials and Methods. DOB, delta over baseline. (B) A total of 26 pathways were enriched by both the differential metabolites and microbiota after successful eradication. Among them, 5 pathways were significantly changed by eradication in both metabolomics and microbiome analyses, including pathways for leishmaniasis, adipocytokine signaling, lipid and atherosclerosis, choline metabolism in cancer, and cholesterol metabolism (P values <0.05 for all). *, P values < 0.05; **, P values < 0.01.