FIG. 3.

PRV entry is sensitive to gD-mediated interference. CHO cells cotransfected with mHveB-expressing plasmid (pDS6) and gD-expressing plasmids [pRE4 for wild-type HSV-1 gD (9), pMW13 for mutant HSV-1(KOS)Rid1 gD (32), pCMV-gD for PRV gD (13), and pcDNA3 as control] in a 1:1 ratio (A to C) or a 1:4 ratio (D to F) were infected with β-galactosidase-expressing PRV at the doses indicated. Six hours later, the cells were rinsed and solubilized by addition of 0.5% Nonidet P-40 in phosphate-buffered saline containing ONPG (o-nitrophenyl-β-d-galactopyranoside), and the enzymatic activity was monitored at 410 nm.