FIG. 4.

Expression of mHveB mRNA in mouse cell lines. Total RNA was isolated from the cell lines indicated, and reverse transcription-PCR was performed as described in the text, generating a 494-bp fragment from mHveB cDNA and a 329-bp fragment from β₂-microglobulin cDNA. The primers for mHveB were 5′-AGAGTGGAACACGAGAGCTT-3′ and 5′-GGATCCTCTGTCGCCATCAT-3′, and those for β₂-microglobulin were 5′-GACCCTGGTCTTTCTGGTGC-3′ and 5′-AGTAGACGGTCTTGGGCTCG-3′ (30). (Upper panel) mHveB expression in mouse cell lines Neuro-2a (neuroblastoma), K1735 (melanoma), NIH 3T3 (fibroblast), SJKY (fibroblast), P388D1 (macrophage), A20 (B-cell lymphoma), BCL1 (B-cell lymphoma), LM-tk⁻ (connective tissue), NJ101 (B-cell lymphoma), B16 (melanoma), and B78H1 (melanoma) plus or minus reverse transcriptase. SJKY, P388D1, A20, BCL1 and NJ101 were obtained from B. Kim; B16, B78H1, and K1735 were obtained from N. Fraser; and Neuro-2a, LM-tk⁻, and NIH 3T3 were obtained from the American Type Culture Collection. (Lower panel) β₂-Microglobulin expression, plus or minus reverse transcriptase.