FIG. 5.

Infection of mouse melanoma (B78H1) cells is dependent on mHveB. B78H1 cells were preincubated with anti-mHveB MAb (squares) or control anti-myc MAb (circles), and NJ101 cells were preincubated with anti-mHveB MAb (diamonds). After 30 min of incubation, the cells were challenged by the addition of a constant dose of β-galactosidase-expressing PRV. After 3 h of incubation, the virus-MAb mixtures were removed, unpenetrated virus was inactivated by brief treatment with 100 mM citrate buffer (pH 3.0), and incubation was continued for another 3 h. The cells were permeabilized, and ONPG (o-nitrophenyl-β-d-galactopyranoside) substrate was added for quantitation of β-galactosidase activity as described in the text.