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. 2023 Aug 7;120(33):e2305420120. doi: 10.1073/pnas.2305420120

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Copyright © 2023 the Author(s). Published by PNAS.

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Fig. 3. — BB-Cl-amidine inhibits STING signaling independent of PADs. (A) qPCR analysis of Ifnβ expression in WT and Padi4^−/− BMDMs treated with diABZI-4 for 2 h. (B) Immunoblot analysis of phosphorylated IRF3, STING, and PAD4 in whole-cell lysates from WT and Padi4^−/− BMDMs treated with diABZI-4 for the indicated times. (C and D) qPCR analysis of Ifnβ (C) and Cxcl0 (D) expression in BMDMs pretreated with 1 μM BB-Cl-amidine for 1 h followed by treatment with diABZI-4 for 2 h. (E) Immunoblot analysis of phosphorylated IRF3, PAD4, and β-actin in whole-cell lysates from WT and Padi4^−/− BMDMs pretreated with 1 μM BB-Cl-amidine for 1 h followed by treatment with diABZI-4 for 1 h. (F and G) qPCR analysis of Ifnβ (F) and Cxcl0 (G) expression in WT, Padi2^−/− and Padi2/4^−/− BMDMs treated with diABZI-4 for 2 h. ***P < 0.0001. Error bars show mean ± SEM.