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PEA counteracts immune cell infiltration caused by HFD overfeeding. (A) Protein levels of TLR4 were evaluated by Western Blot analysis. (B–F) PEA normalized mRNA levels of Itgax, Emr1, Cma1, Tpsb2 and Crh in the colon of HFD group (n = 5–6 each group). A representative Western blot is shown for TLR4. Data are presented as mean ± SEM reaching the significance at P < 0.05 (*P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001).
