Figure 2.

Quantification (A) and representative immunofluorescence imaging (B, C) of CD45+ cells (red) in the corneas of wild-type or gene-knockout mice in TRPA1 (−/−) or TRPV1 (−/−) with or without contact lens wear for 24 hours. Corneal nerves were labeled with β-tubulin III (green). Scale bar = 70 µm. CD45+ cell responses to lens wear (white bars) were present in all mice: WT, TRPA1 (−/−), and TRPV1 (−/−). Contralateral eyes of TRPA1 (−/−) and TRPV1 (−/−) mice (grey bars) did not show a significant reduction in baseline numbers of CD45+ cells * P < 0.05, *** P = 0.001, ns = not significant [One-way ANOVA with Tukey's multiple comparisons test].