Figure 7.

(A) Immunofluorescence imaging of corneal cryosections showing Ly6G+ cell (neutrophil) infiltration of a mouse cornea after 6 days of contact lens wear versus the no lens wear contralateral eye with and without RTX treatment to deplete TRPV1 nociceptors. RTX treatment attenuated the lens-induced Ly6G+ cell infiltration. Ly6G+ cells (green), cell nuclei (blue), and cell F-actin (red). Scale bar = 30 µm. (B) Quantification of Ly6G+ cells (per field of view) in the corneas of WT mice treated with PBS (control) or RTX with and without 6 days of contact lens wear. RTX treatment prevented lens-induced Ly6G+ cell infiltration. **** P < 0.0001, ns = not significant [One-way ANOVA with Tukey's multiple comparisons test].