Figure 1.

The majority of individual LH^Vgat neurons show inconsistent responding to water and sucrose drinking, both during chow and fcHFD feeding. (A) Schematic of experimental design, per imaging day there was a maximum of 60 trials, with 3 recording days per stimulus (water/sucrose). (B) Mean intensity map of an example field of view during imaging all included ROIs outlined, numbers indicate the 10 neurons plotted in (C). (C) Extracted individual calcium (ΔF/F) traces per neuron showed in (B). (D) Example heatmaps of individual neurons (over 10 trials) showing a neuron labeled as a “consistently negative” responder (left), a “consistently positive” responder (middle) and an “inconsistent” responder (right). Consistently responding was considered more than half of the trials a significant response (average ΔF/F > 0.05 or <−0.05). (E) Percentages of labeled neurons per diet group (chow/fcHFD) and consumed drink (water/sucrose). During chow feeding, the number of neurons showing a consistently positive response were increased upon sugar drinking compared to water (χ² 4.29, p: 0.03*). During fcHFD feeding, number of consistently negative responding neurons lowered upon sugar drinking compared to water (χ² 5.81, p: 0.02*).