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. 2023 Aug 7;19(8):e1011274. doi: 10.1371/journal.ppat.1011274

Fig 3. ZIKV infection in the decidua, trophoblastic shell, and chorionic membrane at 7 dpi.

Fig 3

ZIKV infection was seen in (A)–(C) a decidual vessel, (D)–(F) decidual macrophages, (G)(H) in the trophoblastic shell, and (I–K) chorionic membrane. (A) H&E staining of the section shown in (B) Pink staining shows ZIKV RNA detected via ISH (C) IHC staining for cytokeratin. (D) H&E staining of the corresponding section shown in (E). (E) ISH (pink) staining showing ZIKV RNA in the decidua. (F) IF staining for CD163 (red) to identify macrophages, ZIKV (green), and DAPI nuclear staining (blue). Colocalization of CD163, ZIKV, and DAPI is outlined by the dashed circles. The scale bar represents 200 μm. (G) Pink staining shows ZIKV RNA detected via ISH (H) IHC staining for cytokeratin, highlights the trophoblastic shell. (I) H&E staining of the section shown in (J). (J) Pink staining showing ZIKV RNA in the chorionic membrane. (K) IHC staining for cytokeratin identifies the trophoblast layer of the chorionic membrane. (A)–(E), (G)–(K) Scale bars represent 500 μm.