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. 2023 Aug 7;19(8):e1011274. doi: 10.1371/journal.ppat.1011274

Fig 7. Infection in the placental villi at 14 dpi.

Fig 7

(A) Pink staining shows ZIKV detected via ISH. (C) corresponding H&E section. The red square in (A) identifies the area magnified in (B) as does the black square in (C) for (D). (A)(C) The scale bars represent 500 μm. (B)(D) The scale bars represent 100 μm. (E) IF staining for CD163 (red) ZIKV (green) and DAPI (blue). The scale bar represents 400 μm. The orange square shows the portion that is magnified in (F). (G) IF staining for cytokeratin (red) ZIKV (green) and DAPI (blue). (E)(G)The scale bars represent 400 μm. (H) Multiplex fluorescence in situ hybridization (mFISH) to detect genomic, positive sense ZIKV RNA (green) and replicative intermediate, negative sense RNA (red) with nuclear DAPI staining (blue). The scale bar represents 50 μm. The replicative intermediate negative sense RNA (red) is shown alone in (I).