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. 2023 Aug 7;19(8):e1011274. doi: 10.1371/journal.ppat.1011274

Fig 9. ZIKV infection in large decidual vessels at 6 dpi.

Fig 9

(A) Photomicrograph of a representative coronal section of the gravid uterus evaluated via ISH for ZIKV RNA (pink). Pink tissue/structure names indicate the detection of ZIKV RNA via ISH. (B) Magnification of ZIKV RNA detected in a decidual vessel. (C) H&E staining of a serial section. (D) IF staining of the same decidual vessel for cytokeratin (red), ZIKV (green), and DAPI for nuclear staining (blue). (E) Multiplex fluorescence in situ hybridization (mFISH) to detect genomic, positive sense ZIKV RNA (green) and replicative intermediate negative sense RNA (red) with nuclear DAPI staining (blue). Colocalization of genomic RNA and replicating RNA (yellow) showing that the ZIKV RNA detected in the EVTs represents replicating virus. (B)–(E) The scale bars represent 200 μm.