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. 2023 Aug 7;19(8):e1011274. doi: 10.1371/journal.ppat.1011274

Fig 12. Mesenchymal tissue of the chorionic plate and villi infected with ZIKV at 10 dpi.

Fig 12

(A) full slide evaluated with ISH shown as pink staining. The orange square outlines the area of the slide magnified in portion (B). (B) CP indicates chorionic plate. The black square outlines the portion of the slide magnified in (C–E). (D) H&E of the corresponding section is shown in (C). (C)(D) Arrows indicate endothelial cells, m indicates mesenchymal tissue and t indicates trophoblasts in the placenta. (E) Corresponding section with IHC staining for CD163. (F)(G) IF staining for CD163 (red) ZIKV (green) and DAPI nuclear staining (blue). (F) Photomicrograph of the placental villi with colocalization of green and red showing that ZIKV is within Hofbauer cells in the placenta villi, but not exclusively. (G) Depicts the chorionic plate of the placenta where ZIKV almost exclusively co-localized with CD163 showing ZIKV predominantly within macrophages in the chorionic plate. Scale bars represent 200 μm in (B)–(F) and 400 μm in (G).