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. 2023 Aug 17;12:RP87283. doi: 10.7554/eLife.87283

Figure 1. Identification of two novel C-terminal short forms of METTL3.

(a) Immunoblot of lysates from paired breast cancer patient non-tumor (N) and tumor (T) tissues. (b) Immunoblot of T47D cells infected with lentivirus encoding METTL3 shRNA or control shRNA by antibodies recognizing N- or C-terminal of METTL3 as indicated. (c) Immunoblot of T47D cells infected with retrovirus encoding shMETTL3 resistant pMSCV-METTL3 without tag or control vector followed by METTL3 shRNA or control shRNA infection. (d) Immunoblot of T47D cells infected with retrovirus encoding pMSCV-METTL3 with C-terminal Flag tag. (e) Purified recombinant METTL3-His protein was incubated with or without T47D cell lysates at 37°C for 1 hr followed by immunoblot with anti-His antibody. (f) Ponceau S staining and the N-terminal sequence of the short forms of METTL3 determined by Edman sequencing are shown. FL indicates the full-length of METTL3. The short forms are labeled as a and b.

Figure 1—source data 1. Unedited western blot images for Figure 1.

Figure 1.

Figure 1—figure supplement 1. The short forms bands exist in lung cancer cells and other cell lines.

Figure 1—figure supplement 1.

Immunoblot of lysates from lung cancer cells (a) or renal cancer cells (b) or HCT116 and MEF cells (c).
Figure 1—figure supplement 1—source data 1. Unedited western blot images for Figure 1—figure supplement 1.