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. 2023 Aug 17;12:RP87283. doi: 10.7554/eLife.87283

Figure 3. METTL3a contributes to cell proliferation and breast cancer progression.

Figure 3.

Immunoblot (a) and cell proliferation (b) of T47D cells infected with retrovirus encoding sgMETTL3 resistant METTL3 variants or control vector (Vec) followed by another infection with sgMETTL3 or sgControl (sgCtrl). Immunoblot (c) and cell proliferation (d) of MDA-MB-231 cells infected with retrovirus encoding sgMETTL3 resistant METTL3 variants or control vector (Vec) followed by another infection with sgMETTL3 or sgControl (sgCtrl). Immunoblot (e) and cell proliferation (f) of 293T cells with WT or CRISPR knock-in mediated deletion of Q238. Immunoblot analysis (g) and cell proliferation (h) of Δ238 knock-in 293T cells rescued with METTL3a or METTL3b. (i–k) Mouse xenograft experiments were performed with MDA-MB-231 cells infected with retrovirus encoding sgMETTL3 resistant METTL3-WT, METTL3-Δ198, or METTL3-Δ238 followed by another infection with sgMETTL3 (n = 8 mice per group). Tumor growth curve (i), tumor (j), and tumor weight (k) were recorded. Error bars represent mean ± standard error of the mean (SEM), unpaired t-test. ***p < 0.001, ns denotes no significance. FL indicates the full-length of METTL3. The short forms are labeled as a and b.

Figure 3—source data 1. Unedited western blot images for Figure 3a, c, e, and g and picture related to Figure 3j.
Figure 3—source data 2. Tables related to Figure 3b, d, f, h, i, and k.