Figure 20. CaN temperature changes in our model caused by 1Pre, 100 at 20 Hz with glutamate uncaging (no failures allowed), 2 mM Ca, Mg-free, 11–13 days in vitro.

(a), Simulated CaN change (blue solid line) in response to the same stimuli of the CaN measurement from Fujii et al., 2013 RY-CaN fluorescent probe (green solid line). The decay time ($\tau$) estimated from data ($y\mathrm{=}a\mathrm{\cdot }{e}^{\mathrm{-}t\mathrm{\cdot }b}$) is 94.83 s (dashed purple line) and 82.66 s for our model (solid purple line). (b), Simulated CaN change for physiological temperature with decay time of 54.44 s. (c), Temperature change, ${\rho }_f^{C\mathbf{}a\mathbf{}N}$ and ${\rho }_b^{C\mathbf{}a\mathbf{}N}$, applied to CaN association and dissociation rates.