Table 6. Postsynaptic calcium dynamics parameters.

Note that the buffer concentration, calcium diffusion coefficient, calcium diffusion time constant and calcium permeability were considered free parameters to adjust the calcium dynamics.

Name	Value	Reference
Buffer and dye
Association buffer constant	$k_{on}^{Buff}=247\mu M^{-1}{s}^{-1}$	Bartol et al., 2015
Dissociation buffer constant	$k_{off}^{Buff}=524s^{-1}$	Bartol et al., 2015
Generic buffer concentration	$Buf{f}_{con}=62\mu M$	$76.7\mu M$ (Bartol et al., 2015)
Calcium dynamics
Calcium baseline concentration	$C{a}_{\mathrm{\infty }}=0.05\mu M$	${\displaystyle 0.037\pm 0.005\text{ to }0.054\pm 0.005\mu M}$  (Maravall et al., 2000)
Calcium decay time	${\tau }_{Ca}={10}^{-2}s$	0.05–0.5 s with dye (Maravall et al., 2000) therefore < 0.05–0.5 s undyed (unbuffered)
Calcium diffusion coefficient	$D_{Ca}=333.8\mu m^2{s}^{-1}$	${\displaystyle 200\text{ to }400\mu m^2{s}^{-1}}$ (Bartol et al., 2015; Holcman et al., 2005)
Calcium diffusion time constant	${\displaystyle {\tau }_{CaDiff}=\frac{Vo{l}_{sp}}{2D_{Ca}^2\cdot D_{neck}}+\frac{L_{neck}^2}{2D_{Ca}}=5\cdot {10}^{-4}s}$	${\displaystyle 8ms\text{ for a }}$  $Vo{l}_{sp}=0.7\mu m^3$ (Holcman et al., 2005)
GHK equation
Temperature	$T={35}^{\circ }C$	converted to Kelvin in the Equation 14 given the protocol
Faraday constant	$F=96.485Cmo{l}^{-1}$	Hille, 1978
Gas constant	$R=8.314J{K}^{-1}mo{l}^{-1}$	Hille, 1978
Calcium permeability	${\displaystyle P_{Ca}=45\mu m{s}^{-1}}$	adjusted to produce 3μM Calcium in response to a Glu release supplementary file from Chang et al., 2017
Calcium ion valence	$z_{Ca}=2$	Hille, 1978