Figure 5.

Sting depletion partially rescues histological defects in the seminiferous tubules in Fancm-deficient mice

(A) H&E stain of seminiferous tubules in B6.Fancm^+/+ and B6.Fancm^Δ2/Δ2 mice. Scale bar, 100 μm. Wild-type tubules are relatively replete in their appearance. Mutant mice have a heterogeneous reduction in spermatocytes (hypospermatogenesis) at the level of individual tubules. A mixture of normal, atrophied, symplast-positive, and Sertoli cell-only tubules is observed.

(B) Fancm^Δ2/Δ2Sting^−/− testes have more full tubules than the Fancm single mutant (p-adjusted < 0.0001). Quantification of the PAS-stained seminiferous tubule cross sections. Each data point represents data from one mouse shown as a proportion for the given classification (∗p $\le$ 0.05, ∗∗p $\le$ 0.01, ∗∗∗∗p $\le$ 0.0001 (pairwise proportion test with multiple testing correction fdr). A total of 290 Fancm^+/+Sting^+/+, 305 Fancm^+/+Sting^−/−, 479 Fancm^Δ2/Δ2Sting^+/+, and 605 Fancm^Δ2/Δ2Sting^−/− tubules were analyzed. Gray bars indicate mean ± SD.