REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Rabbit polyclonal FANCD2	Novus Biologicals	NB100-182; RRID:AB_10002867
Mouse ɑ-tubulin	Cell Signaling	#3873; RRID:AB_1904178
Cleaved caspase-3	Cell Signaling	#9664; RRID:AB_2070042
Cleaved caspase-7	Cell Signaling	#9491; RRID:AB_2068144
Deposited data
Single-cell DNA sequencing of sperm cells and bulk DNA sequencing of BC1F1 pups	This paper	European Nucleotide Archive https://www.ebi.ac.uk/ena/browser/home ENA: PRJEB57095
Analysis pipelines and processed data files	This paper	https://doi.org/10.5281/zenodo.7939158
FVB/N SNP variants	Mouse Genome Project https://www.sanger.ac.uk/data/mouse-genomes-project/	https://ftp.ebi.ac.uk/pub/databases/mousegenomes/REL-1505-SNPs_Indels/strain_specific_vcfs/
Mouse reference genome Genome Reference Consortium Mouse Build 38 (mm10)	Genome Reference Consortium	https://www.ncbi.nlm.nih.gov/assembly/GCF_000001635.20/
GC Percent track GRCm38	University of California, Santa Cruz	https://hgdownload.soe.ucsc.edu/goldenPath/mm10/bigZips/
RepeatMasker	Smit et al.135	http://www.repeatmasker.org
Experimental models: Organisms/strains
Fancm C57BL/6J	This paper	N/A
Fancm FVB/N	This paper	N/A
C57BL/6J	This paper	N/A
FVB/N	This paper	N/A
Oligonucleotides
Fancm forward primer 5′ CGGGGCGG AATGCTAAACTT 3′	This paper	N/A
Fancm reverse primer #1 5′ ACACACA GGGACAGAGAACACTC 3′	This paper	N/A
Fancm reverse primer #2 5′ GGAAAAG AGAAAAGAAAAGGGGGA 3′	This paper	N/A
Sting forward primer #1 5′ GCTGGGAA TTGAACGTAGGA 3′	This paper	N/A
Sting forward primer #2 5′ 3′	This paper	N/A
Sting reverse primer 5′ GAGGAGACAA AGGCAAGCAC 3′	This paper	N/A
Software and algorithms
R (3.5.1)	R Core Team, 2021	https://www.r-project.org/
lme4	Bates et al.136	https://cran.r-project.org/web/packages/lme4/index.html
cellranger-dna cnv (v1.1.0)	10X Genomics	https://support.10xgenomics.com/single-cell-dna/software/pipelines/latest/using/cnv
glmmTMB	Brooks et al.137	https://cran.r-project.org/web/packages/glmmTMB/index.html
sgcocaller and comapr	Lyu et al.86	https://gitlab.svi.edu.au/biocellgen-public/sgcocaller https://bioconductor.org/packages/release/bioc/html/comapr.html
Gviz	Hahne et al.138	https://bioconductor.org/packages/release/bioc/html/Gviz.html
biomRt	Durinck et al.139	http://www.bioconductor.org/packages/release/bioc/html/biomaRt.html
Fastp	Chen et al.140	https://github.com/OpenGene/fastp
minimap2-v2.7	Li, Heng141	https://github.com/lh3/minimap2
gatk(v4.0)	McKenna et al.142	https://gatk.broadinstitute.org/hc/en-us
code repository	This paper	https://gitlab.svi.edu.au/biocellgen-public/fancm-crossovers-2022
Fiji (ImageJ-win64)	Schindelin et al.143	https://fiji.sc/
ImageJ Scripts for axis detection and foci analysis	Boekhout et al.144	https://github.com/Boekhout/ImageJScripts
synapsis	McNeill, L.	http://bioconductor.org/packages/release/bioc/html/synapsis.html