FIG. 1.
Isolation and characterization of a TBP mutant that complements a TBP mutant with a Pol III defect. (A) Growth conferred by the indicated TBP derivatives and wild-type (wt) TBP at both 30 and 38°C. Cells were grown overnight in liquid medium, and approximately 105 cells were spotted onto the medium lacking the appropriate markers. (B) Growth conferred by TBP derivatives singly substituted at position 151 or 156. (C) S1 nuclease analysis of tRNAW transcription with 30 μg of RNA from the indicated TBP derivative after shifting cultures to 38°C for 1 h. (D) Mutant and wild-type strains containing PNOY103 (see text) were grown overnight in liquid medium (2% galactose), spotted to galactose medium, and incubated at 30 and 38°C. (E) S1 nuclease analysis of an unstable portion of the rRNA transcript with 70 μg of RNA from the indicated strains after incubation at 30 or 38°C for one hour.