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. 2023 Aug 17;14:5004. doi: 10.1038/s41467-023-40705-z

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a Three-dimensional (3D) representation of the detailed interactions between MRGPRX1 and the α5-helix of Gαi. b Three-dimensional (3D) representation of the detailed interactions between MRGPRX1 and the α5-helix of Gαq. c Comparison of the Gi1/Gq coupling interfaces in cryo-EM structures of BAM8-22-MRGPRX1-Gq, BAM8-22-MRGPRX1-Gi1 and PAMP-12-MRGPRX2-Gi1 complexes. Residues of MRGPRX1 in contact with Gi1/Gq were illustrated as green dots. d Effects of mutations in the MRGPRX1 along the Gi trimer interface on BAM8-22 induced Gαi-Gγ dissociation. Statistical differences between WT and mutations were determined by two-sided one-way ANOVA with the Tukey test. *P < 0.05; **P < 0.01; ***P < 0.001, ns, no significant difference. (P = 0.0109, 0.0091, ND, ND, ND, 0.1196, <0.001, ND from top to bottom). Data from three independent experiments are presented as the mean ± SEM (n = 3). e The structural representation and comparison of the interfaces between the MRGPRX1-Gi and MRGPRX1-Gq complexes. Ribbon representation: MRGPRX1 bound to Gi is shown in cornflower blue, MRGPRX1 bound to Gq is shown in salmon, Gi is shown in dark orange, Gq is shown in green. f Effects of mutations in the MRGPRX1 along the Gαq trimer interface on BAM8-22 induced Gαq-Gγ protein dissociation. Statistical differences between WT and mutations were determined by two-sided one-way ANOVA with the Tukey test. *P < 0.05; **P < 0.01; ***P < 0.001, ns, no significant difference. (P = 0.0003, <0.001, <0.0004, <0.001, <0.001, <0.001, <0.001, P < 0.001, ND, ND, <0.0001 from top to bottom) Data from three independent experiments are presented as the mean ± SEM (n = 3).