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. 2023 Aug 18;13(1):124. doi: 10.1038/s41408-023-00899-3

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — A The tSNE and dot plots from a representative FL biopsy specimen showing expression of CD19 and CCR6 in CD45⁺ cells. Graphs showing percentage of CD19⁺CCCR6⁺ cells from 4 tonsil tissue and 82 FL specimens. B The tSNE plots of CD45⁺ cells showing expression of CD27, CCR7, CD45RA, HLA-DR, and CD21 from a representative specimen. Graphs showing mean fluorescent intensity (MFI) of surface markers above in CD19⁺CCR6⁺ and CD19⁺CCR6⁻ cells. C The tSNE plots of CD45⁺ cells showing expression of CCR6, CD27, CD7, CD28 and CD5 from patients with low or high number of CCR6⁺ cells. Graphs showing percentage of surface markers above in patients with low or high number of CCR6⁺ cells. D CITRUS plot showing clustering result from FL patients between patients with low or high number of CCR6⁺ cells. Circles in red represent clusters that differed between groups and number in circles indicates a cluster ID (i). Histogram plots showing expression of selected markers by cells from clusters overlaid on background staining (ii). Graph showing quantitative results of abundance from clusters (iii). E Volcano plots showing genes (dots) of upregulation (red), downregulation (blue) or no change (black) from CITE-seq analysis based on Log2 FC and adjusted p value. P < 0.05 indicates statistically significant. F The tSNE plot showing clusters from CD19⁺CCR6⁻ and CD19⁺CCR6⁺ cells based on gene expression profile from CITE-seq analysis. Heatmap showing gene expression by clusters from the tSNE plot. Representative genes were listed with cluster number in red. G The tSNE plots showing expression of genes TCL1A, FCMR, CD72, LY6, IGKV1D-8, SGK1, DYNLL1, and MFHAS1 from CD19⁺CCR6⁻ and CD19⁺CCR6⁺ cells in FL biopsy specimens.