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. 2023 Aug 17;14:4974. doi: 10.1038/s41467-023-40743-7

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Schematic of in utero electroporation (i.u.e.) procedure (up) and experimental timeline (bottom). b Expression of L1 transcripts in GFP⁺ cells from E14.5 cortices i.u.e with shCtrl or shL1-a. RNA levels are normalized on shCtrl group. Data are mean ± s.e.m. n = 3 technical replicates. Two-sided unpaired t-test. c, e Immunofluorescence staining for neuronal progenitor (Pax6) and pro-neuronal (NeuroD1) markers at E13.5 (c) and E14.5 (e). d, f Quantification of GFP⁺/marker⁺ cells at E13.5 (d) and E14.5 (f). Data are mean ± s.e.m.; E13.5: n = 5 each; E14.5: n = 4 shCtrl, n = 5 shL1-a. Two-sided unpaired t-test. g Immunofluorescence staining for DAPI and GFP at E18.5. h Radial distribution of GFP⁺ cells at E18.5. Data are mean ± s.e.m.; n = 4 shCtrl, n = 3 shL1-a. Multiple t-test with correction for multiple comparison with Sidak-Bonferroni’s test. i, j Top GO terms under the biological process category for up-regulated (i) and down-regulated (j) genes in mouse GFP⁺ cells after i.u.e. of shL1-a versus shCtrl. p values were determined by gprofiler2 using a default hypergeometric test and correction for multiple testing has been performed by the g:SCS algorithm. Source data are provided as a Source Data file.