Fig. 2.

Effects of central administration of NTI on the Kiss1 (kisspeptin gene) expression in the arcuate nucleus (ARC) of OVX lactating rats during early lactation. Kiss1-expressing cells in the ARC in representative OVX lactating rats treated with vehicle or NTI in the 3V 1 h before brain sampling (A). The insets indicate Kiss1-expressing cells (black arrowheads) at higher magnification. NTI administration did not affect the intensity of Kiss1 mRNA signals and the number of Kiss1-expressing cells in the ARC of OVX lactating rats (B). Scale bars = 100 µm. Values are means ± SEM. The number in each column indicates the number of animals used.