FIGURE 3.

Sanger sequencing of the 185 bp size PCR products obtained from a polyacrylamide gel. Nucleotide sequences of wild‐type TET2 transcript are shown at the bottom of each chromatogram. The coding exon sequences were highlighted in blue. The splicing junctions connecting exon 7 and exon 8, leading to partial depletion of exon 7, are indicated with black arrows. (A) Chromatogram of patient 1 fragment of TET2 c.3954+5_3954+8del. (B) Chromatogram of patient 2 fragment of c.3954+5G>A substitution.