FIG. 2.
The SRF promoter directs transient expression of a human c-fos gene reporter. (A) Schematic representation of derivation of the SRF–c-fos chimeric reporter. (B) NIH 3T3 fibroblasts were transfected with −322SRF/c-fos and serum starved. Cells were serum stimulated for the times (minutes) indicated, and total RNA was isolated. RNase protection assays were performed. Data were quantitated by PhosphorImager analysis. Levels of expression of the −322SRF/c-fos reporter were normalized for transfection efficiency against the level of expression of the transfected α-globin gene. Positions of the protected RNA species are indicated (transfected SRF-human c-fos [srf-fosH] = 251 nt; globin = 133 nt; endogenous mouse c-fos [fosM] = 65 nt).