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. 2023 Aug 17;222(10):e202112143. doi: 10.1083/jcb.202112143

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© 2023 Jones et al.

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Figure 6. — Localization of aPKC with PB1 domain perturbations in larval brain NSCs. (A) Schematics of D77A and ∆PB1 aPKC variants. (B) Localization of HA-tagged aPKC D77A and ∆PB1 variants in metaphase larval brain NSCs. The basal marker Miranda, and total aPKC (expressed variant and endogenous), are shown for comparison. Scale bar is 5 µm. (C and D) Gardner-Altman estimation plots of aPKC D77A and ∆PB1 cortical localization. Apical cortical to cytoplasmic (C) or apical to basal (D) signal intensity ratios of anti-HA signals are shown for individual metaphase NSCs expressing either aPKC D77A or ∆PB1. The data for wild type is the same as in Fig. 1. Apical to basal ratios are only shown for proteins with detectable membrane signal. The error bar in the upper graphs represents one standard deviation (gap is mean); the error bar in lower graphs represents bootstrap 95% confidence interval; n = 16 (from six distinct larval brains), 4 (1), and 6 (4) for WT, D77A, and ∆PB1, respectively.