Table 1.
Key resources table
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Antibody | Anti-aPKC | SCBT | Mouse Anti-PKC zeta (H1); SC-17781 | 1:1,000 |
| Antibody | Anti-Par-6 | Alpha diagnostic | N/A | Rat Anti-Par-6 (polyclonal custom antibody); 1:500 |
| Antibody | Anti-Mira | Abcam | Rat Anti-Mira; Ab197788 | 1:500 |
| Antibody | Anti-HA | Cell Signaling Tech. | Rabbit Anti-HA (C29F4); 3724 | 1:1,000 |
| Antibody | Anti-HA | Covance | Mouse Anti-HA; MMS-101P | 1:500 |
| Antibody | Anti-Baz | C.Q.Doe Lab | N/A | Guinea Pig Anti-Baz (polyclonal custom antibody); 1:2,000 |
| Antibody | Anti-GFP | Abcam | Chicken Anti-GFP; Ab13970 | 1:500 |
| Antibody | Anti-rat Cy3 secondary | Jackson Immuoresearch Lab. | Donkey Anti-Rat Cy3; 712-165-153 | 1:500 |
| Antibody | Anti-rabbit 647 secondary | Jackson Immunoresearch Lab. | Donkey Anti-Rabbit 647; 711-605-152 | 1:500 |
| Antibody | Anti-mouse 647 secondary | Jackson Immunoresearch Lab. | Donkey Anti-Mouse 647; 715-605-151 | 1:500 |
| Antibody | Anti-mouse 488 secondary | Jackson Immunoresearch Lab. | Donkey Anti-Mouse 488; 715-545-151 | 1:500 |
| Antibody | Anti-chicken 488 secondary | Jackson Immunoresearch Lab. | Donkey Anti-Chicken 488; 703-545-155 | 1:500 |
| Antibody | Anti-guinea pig 405 secondary | Jackson Immunoresearch Lab. | Donkey Anti-Guinea Pig 405; 706-475-148 | 1:500 |
| Genetic reagent (Drosophila melanogaster) | insc-GAL4 | Bloomington Drosophila Stock Center | ;insc-GAL4 | RRID:BDSC_8751 |
| Genetic reagent (Drosophila melanogaster) | elav-GAL4 | Bloomington Drosophila Stock Center | elav-Gal4, UAS-mCD8:GFP, hs:flp; FRT-G13, tubPGal80 | RRID:BDSC_5145 |
| Genetic reagent (Drosophila melanogaster) | c855a-Gal4 | Bloomington Drosophila Stock Center | ;;c855a-GAL4 | RRID:BDSC_6990 |
| Genetic reagent (Drosophila melanogaster) | aPKCK06403 | C.Q. Doe Lab | ; FRT-G13, aPKCK06403/CyO | |
| Genetic reagent (Drosophila melanogaster) | aPKC D388A | This study | ;;3xHA-aPKC D388A (aPKC-PA) | |
| Genetic reagent (Drosophila melanogaster) | aPKC D388A | This study | ;;1xHA-aPKC D388A (aPKC-PA) | |
| Genetic reagent (Drosophila melanogaster) | aPKC WT | This study | ;;1xHA-aPKC 1-606 (aPKC-PA) | |
| Genetic reagent (Drosophila melanogaster) | aPKC K293W | This study | ;;1xHA-aPKC K293W (aPKC-PA) | |
| Genetic reagent (Drosophila melanogaster) | aPKC PB1-C1 | This study | ;;1xHA-aPKC 1-195 (aPKC-PA) | |
| Genetic reagent (Drosophila melanogaster) | aPKC PB1-PS | This study | ;;1xHA-aPKC 1-141 (aPKC-PA) | |
| Genetic reagent (Drosophila melanogaster) | aPKC C1 | This study | ;;1xHA-aPKC 139-195 (aPKC-PA) | |
| Genetic reagent (Drosophila melanogaster) | aPKC ∆C1 | This study | ;;1xHA-aPKC 1-606 ∆141-196 (aPKC-PA) | |
| Genetic reagent (Drosophila melanogaster) | aPKC AADAA | This study | ;;1xHA-aPKC R131A, R132A, A134D, R135A, R136A (aPKC-PA) | |
| Genetic reagent (Drosophila melanogaster) | aPKC ∆PB1 | This study | ;;1xHA-aPKC 107-606 (aPKC-PA) | |
| Genetic reagent (Drosophila melanogaster) | aPKC D77A | This study | ;;1xHA-aPKC D77A (aPKC-PA) | |
| Genetic reagent (Drosophila melanogaster) | Baz RNAi | Bloomington Drosophila Stock Center | ;UAS-Baz RNAi | RRID:BDSC_39072 |
| Genetic reagent (Drosophila melanogaster) | Cdc42 RNAi | Bloomington Drosophila Stock Center | ;UAS-Cdc42 RNAi | RRID:VDRC_100794 |
| Chemical compound, drug | Amylose resin | NEB | E8021L | |
| Chemical compound, drug | Schneider’s Insect Medium (SIM) | Sigma-Aldrich | S0146 | |
| Chemical compound, drug | SlowFade Diamond Antifade Mountant with DAPI | Invitrogen | S36964 | |
| Chemical compound, drug | Phosphatidylserine (PS) | Avanti Polar Lipids | L-α-phosphatidylserine; 840032C | |
| Chemical compound, drug | Phosphatidylcholine (PC) | Avanti Polar Lipids | L-α-phosphatidylcholine; 840051C | |
| Chemical compound, drug | Phosphatidic acid (PA) | Avanti Polar Lipids | L-α-phosphatidic acid; 840101C | |
| Chemical compound, drug | Phosphatidylglycerol (PG) | Avanti Polar Lipids | L-α-phosphatidylglycerol; 841138P | |
| Chemical compound, drug | Ceramide | Avanti Polar Lipids | C12 ceramide; 860512P |