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. 2023 Aug 3;17:1192076. doi: 10.3389/fnbeh.2023.1192076

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Copyright © 2023 Jimenez Chavez, Van Doren, Scheldrup, Rivera, Torres-Gonzalez, Herbert, Denning, Khorsandi, Garcia, Castro and Szumlinski.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Depiction of the results of the Sex × Age × Drinking History ANOVAs conducted for behavior in the light dark box shuttle test. No group differences were observed for the latency to enter the light side of the shuttle box on either WD1 (A) [females: adolescents/Adol. H2O (n = 11), EtOH (n = 12); adults H2O (n = 16), EtOH (n = 8); males: adolescent H2O (n = 10), EtOH (n = 12); adults H2O (n = 12), EtOH (n = 12)] or WD30 (B) [females: adolescents H2O (n = 12), EtOH (n = 12); adults H2O (n = 12), EtOH (n = 11); males: adolescent H2O (n = 11), EtOH (n = 12); adults H2O (n = 12), EtOH (n = 12)]. (C) Summary of the results for the time spent in the light side for all groups tested on WD1 [females: adolescents H2O (n = 12), EtOH (n = 11); adults H2O (n = 16), EtOH (n = 8); males: adolescent H2O (n = 12), EtOH (n = 11); adults H2O (n = 12), EtOH (n = 11)]. (D) On WD1, an Age by Drinking History interaction was observed for the time spent in the light side that reflected less time spent by adult binge-drinking (EtOH) mice versus both adult water (H2O) and adolescent (Adol.) EtOH mice. Additionally, adolescent H2O mice spent less time in the light side than their age- matched EtOH counterparts [adolescents: H2O (n = 24), EtOH (n = 22); adults: H2O (n = 28), EtOH (n = 19)]. (E) Also on WD1, we detected a Sex by Age interaction that reflected more time spent on the light side by adult females (F) versus adult males (M), while no sex difference was apparent in adolescent mice. Adolescent males, however, spent more time in the light side compared to the adult males [females: adolescents (n = 23), adults (n = 24); males: adolescents (n = 23), adults (n = 23)]. (F) On WD30, no group differences were detected for the total time spent in the light side of the shuttle box [females: adolescents H2O (n = 12), EtOH (n = 12); adults H2O (n = 11), EtOH (n = 12); males: adolescent H2O (n = 12), EtOH (n = 12); adults H2O (n = 11), EtOH (n = 10). (G) Results for the total number of entries into the light side of the shuttle box test indicated significant interactions on WD1 between Sex by Drinking History and Age by Drinking History [females: adolescents H2O (n = 12), EtOH (n = 12); adults H2O (n = 15), EtOH (n = 8); males: adolescent H2O (n = 12), EtOH (n = 11); adults H2O (n = 11), EtOH (n = 12)]. (H) Follow-up analysis of the Sex by Drinking History interaction revealed that male EtOH mice exhibited more entries into the light side compared to female EtOH mice [females: H2O (n = 27), EtOH (n = 20); males: H2O (n = 23), EtOH (n = 23)]. (I) The Age by Drinking History interaction on WD1 did not reflect any significant effect of EtOH in either age group [adolescents: H2O (n = 24), EtOH (n = 23); adults: H2O (n = 26), EtOH (n = 20)]. (J) On WD30, female mice exhibited a greater number of entries into the light side compared to male mice, irrespective of age or drinking condition [females: adolescents H2O (n = 1)2, EtOH (n = 12); adults H2O (n = 12), EtOH (n = 12); males: adolescent H2O (n = 12), EtOH (n = 12); adults H2O (n = 12), EtOH (n = 12). The data represent the means ± SEMs for the number of mice indicated above. ⁺p < 0.10, Female vs. Male; ^#p < 0.10, adolescents vs. adults.