FIGURE 1.

Diagrams (A–F) depicting the location of sampling frames for cell counting for each region of interest (indicated in bold next to the respective diagram), applied consistently for every brain measured. The coronal images were adapted from the Allen Brain Atlas (https://atlas.brain-map.org/). The outlines of the measuring frames are projected onto the corresponding atlas image (left side), with some key landmarks (right side) and AP coordinates. The areas delineated by dashed lines were excluded from sampling. 3V, third ventricle; ac, anterior commissure; ACC, anterior cingulate cortex; Amy, amygdala; B, Bregma; BNST, bed nucleus of stria terminalis; cc, corpus callosum; CPu, caudate-putamen; EP, entopeduncular nucleus; Hp, hippocampus; Hyp, hypothalamus; LS, lateral septum; MRN, midbrain reticular nucleus; NAc, nucleus accumbens; OT, olfactory tubercle; PAG, periaqueductal gray; PCC, posterior cingulate cortex; RSC, retrosplenial cortex; SC, superior colliculus; SI, substantia innominata; SN, substantia nigra; st, stria terminalis; TH, thalamus; VL, lateral ventricle; VTA, ventral tegmental area. Scale bar: 1 mm.