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. 2023 Aug 3;17:1235047. doi: 10.3389/fnana.2023.1235047

FIGURE 6.

FIGURE 6

(A,B,D,E): Fluorescence microscope images exemplifying the distribution of cells immunolabeled for calbindin (CB) in a subpallial (NAc) and a pallial (RSC) region of control P7 (A,B) and P60 (D,E) mice. The framed areas on the upper right insets are displayed under intermediate magnification (Scale bar: 100 μm). Characteristic cell types of the regions are shown under high magnification (Scale bar: 20 μm) on the lower right insets. Abbreviation on images: ac, anterior commissure; lf, longitudinal fissure. The enclosed graphs (C,F) show the areal density of CB-positive perikarya in several subpallial and pallial regions, comparing VPA-exposed and control animals. For P7 mice, n = 11–12 hemispheres, representing 6 animals (VPA); n = 18–20 hemispheres, representing 10 animals (control). For P60 mice, n = 6 hemispheres, representing 3 animals (VPA); n = 6 hemispheres, representing 3 animals (control). The values are expressed as M ± S.E.M. ***p < 0.001. Abbreviations as in Figure 4E.