Skip to main content
. 2023 Aug 3;14:1201973. doi: 10.3389/fimmu.2023.1201973

Figure 1.

Figure 1

Assessment of IFN-γ response induced by BoHV-4-M-NSP5. PRRSV-1 M/NSP5 specific IFN-γ responses were assessed following one (prime) or two (prime-boost) immunizations of pigs with BoHV-4-M-NSP5, followed by challenge with PRRSV-1 21301/19 (Study 1). Control pigs were immunized twice with WT BoHV-4 vector. Responses were assessed following peptide stimulation using an IFN-γ ELISpot assay. PBMC responses were assessed longitudinally (A). On D63, PBMC responses to stimulation with peptide pools representing M/NSP5 from PRRSV-1 and -2 are compared (B). Responses were additionally assessed in cells isolated from the BAL (C), thymus (D), inguinal lymph nodes (E), and spleen (F). Data are presented as the unstimulated condition-corrected number of IFN-γ spot-forming cells (S-C) per million cells (B-F). Mean data ± SD are shown for (A), whereas for (B–F), data points represent individual pigs, with the median indicated by a horizontal line. *P < 0.05; **P < 0.005; ***P < 0.0005.