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. 2023 Aug 3;14:1201973. doi: 10.3389/fimmu.2023.1201973

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Copyright © 2023 de Brito, Holtham, Roser, Saunders, Wezel, Henderson, Mauch, Sanz-Bernardo, Frossard, Bernard, Lean, Nunez, Gubbins, Suárez, Davison, Francis, Huether, Benchaoui, Salt, Fowler, Jarvis and Graham

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Evaluation of efficacy of BoHV-4-M-NSP5. The ability of BoHV-4-M-NSP5 immunization to confer protection was assessed following challenge infection of pigs with PRRSV-1 21301/19 (Study 1). The negative control group comprised two immunizations with BoHV-4 vector. PRRSV loads in the blood (A) and lungs (B) were inferred by RT-qPCR analysis of serum and BALF samples, respectively, and gross lung lesions (C) and lung histopathology (D; scores for 7 days post-challenge) were scored. Mean data ± SD are shown for (A), whereas for (B–D), data points represent individual pigs, with the median indicated by a horizontal line.