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. 2023 Aug 3;14:1201973. doi: 10.3389/fimmu.2023.1201973

Figure 3.

Figure 3

Characterization of antigen-specific cytokine responses in the blood of pigs after vaccination with BoHV-4-M-NSP5 and PRRSV-1. PRRSV-1 M/NSP5 specific cytokine responses were assessed following one (prime) or two (prime-boost) immunizations of pigs with BoHV-4-M-NSP5 and PRRSV-1 21301/19 (Study 1). Control pigs were immunized twice with the BoHV-4 vector before challenge. The responses of previously cryopreserved PBMCs from D21, D42, and D70 were assessed following peptide (A–F) and live PRRSV-1 21301/19 (G–L) stimulation using flow cytometry. Expression of IFN-γ alone (A, D, G, J), IFN-γ and TNF (B, E, H, K), or TNF alone (C, F, I, L) by CD4 T cells (A–C, G–I) and CD8 T cells (D–F, J–L) are shown as mean unstimulated condition-corrected data ± SD for each vaccine group. *P < 0.05; **P < 0.005; ***P < 0.0005; ****P < 0.0001.