Table 1.
Study 1 immunization groups and schedule.
| Group | Days post-immunization | ||||
|---|---|---|---|---|---|
| 0 | 21 | 42 | 49 | 70 | |
|
A
(Negative control; n = 12) |
WT BoHV-4 vector 109 pfu (i.m.) | WT BoHV-4 vector 109 pfu (i.m.) | 105 TCID50 PRRSV-1 strain 21301/19 (i.n.) | Euthanasia, post-mortem examination, and sample collection (n = 6) | Euthanasia, post-mortem examination, and sample collection (n = 6) |
|
B
(Experimental vaccine, prime only; n = 12)* |
BoHV-4-M-NSP5 109 pfu (i.m.) | Placebo (DMEM) (i.m.) | Euthanasia, post-mortem examination, and sample collection (n = 5) | Euthanasia, post-mortem examination, and sample collection (n = 6) | |
|
C
(Experimental vaccine, prime-boost; n = 12)* |
BoHV-4-M-NSP5 109 pfu (i.m.) | BoHV-4-M-NSP5 109 pfu (i.m.) | Euthanasia, post-mortem examination, and sample collection (n = 5) | Euthanasia, post-mortem examination, and sample collection (n = 6) | |
*Four unvaccinated sentinel animals were co-housed with groups B and C (two/group) and were euthanized on D42.