Fig. 3.
RSV replication in platelets is limited. CD41+CD45− platelets were gated as shown in Fig. 1. Untreated or UV-inactivated RSV-GFP was added to washed platelets from healthy donors (n=2) at a concentration of 0.1 PFUVero per platelet and incubated for up to 2 days. (a) Graph showing the percentage of GFP-positive platelets, potentially indicating expression of virus-encoded genes and thus the initiation of viral replication. Each data point indicates the average of technical duplicates from a single platelet donor. (b) Flow cytometry plots for one representative donor made using FlowJo software.