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Kinetics of TEVs attachment/engulfment by monocytes. Monocytes were incubated with SYTO RNA-labeled TEVs. After washing, the samples were analyzed by FACSCanto. Green autofluorescence of monocytes was used as a control. Data are presented as the mean ± SEM (n=10). *P<0.05, **P<0.01 and ***P<0.001. Data were analyzed using ANOVA with Tukey's post hoc test. TEVs, tumor-derived extracellular vesicles; MFI, mean fluorescence intensity; ns, non-significant.
